Human Physiology deals with the study of functions and mechanisms working within the organ systems. The subject of Physiology is being taught in the first two years of under graduate M.B.B.S course at Shifa College of Medicine. The subject is being taught in an integrated modular curricular system where the function of a system is taught in co- relation with the structural details (Anatomy) and biochemical aspects (Biochemistry). The Department of Physiology has been striving to impart high-quality basic education to its medical students. The goal is to help students develop the ability to think critically and apply basic principles of Physiology to solve problems related to health and disease in Pakistan and to train them as competent physicians and researchers for tomorrow.

The Physiology laboratory is well equipped with the latest and modern apparatus and instruments like Power lab, Spiro lab, Spirometer, Binocular microscopes, Ophthalmoscope, Perimeter, PEF meter, Centrifuge, ECG machine etc. The laboratory is also provided with overhead projector and multimedia facilities to help students benefit maximally from the experimental work.
The Department is also recognized for postgraduate FCPS training in Physiology since 2002. So far ten postgraduate students have passed out, adding to the prestige of the institution. Three FCPS residents are currently undergoing training under the supervision of Dr. Riffat Shafi (Professor and Head of the Department, Physiology). The Department is actively involved in research. This includes the comprehensive research carried out by the postgraduate students as a prerequisite for their FCPS qualification, as well as research carried out by faculty members in the fields of Clinical Physiology and Medical Education. The mission is to promote and establish a research environment, which is conducive to learning and creating new knowledge.